Terminal Transferase from New England Biolabs

Product Specs
ItemTerminal Transferase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0315S
Quantity500 units
Concentration20,000 units/ml
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol dATP into acid-insoluble material in 1 hour at 37°C using d(A)18 as primer.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Using sno-lncRNAs as potential markers for Prader-Willi syndrome diagnosisRNA BiologyDecember 31, 2023Jiu-Ru Sun, Liang-Zhong Yang, Yang-Li Dai, Huang Wu, Siqi Li, Yi-Feng Xu, Youkui Huang, Hao Wu, Zheng Shen, Chaochun Zou, et al.GenScript Company and labelled with Digoxigenin (Dig) using a terminal transferase kit (NEB, Cat. No. M0315). 20 nM complex of gRNA, MhdCas13c, and target RNA were incubated with 200 nM reporter RNA in cleavage

(2) capTEs enables locus-specific dissection of transcriptional outputs from reference and nonreference transposable elementsCommunications BiologySeptember 23, 2023Xuemei Li, Keying Lu, Xiao Chen, Kailing Tu, Dan Xieamplified cDNA in a 50 μL mixture containing 0.1 mM ddGTP (Roche, 03732738001), 0.25 mM CoCl2 (NEB, M0315), 20 U of Terminal Transferase (NEB, M0315) and 1× TdT buffer (NEB, M0315). The reaction was performed

(3) Droplet-based high-throughput single microbe RNA sequencing by smRandom-seqNat CommunAugust 23, 2023Ziye Xu, Yuting Wang, Kuanwei Sheng, Raoul Rosenthal, Nan Liu, Xiaoting Hua, Tianyu Zhang, Jiaye Chen, Mengdi Song, Yuexiao Lv, et al.reaction kit (including TdT enzyme, 10X TdT buffer and CoCl2) was ordered from New England Biolabs (Cat. M0315S). Microfluidic device fabrication PDMS-based microfluidic devices were designed for hydrogel beads

(4) Concurrent profiling of multiscale 3D genome organization and gene expression in single mammalian cellsbioRxivJuly 27, 2023Tianming Zhou, Ruochi Zhang, Deyong Jia, Raymond T. Doty, Adam D. Munday, Daniel Gao, Li Xin, Janis L. Abkowitz, Zhijun Duan, Jian Ma(Thermo Fisher Scientific, R0161), 0.05 μM ddGTP (MilliporeSigma, GE27-2045-01), 0.2 U/μl Terminal Transferase (NEB, M0315L)) and incubated at 37 °C for 20 min. The supernatant was removed by placing the sample on a magnetic

(5) Efficient and sensitive profiling of RNA–protein interactions using TLC-CLIPNucleic Acids ResearchJuly 21, 2023Christina Ernst, Julien Duc, Didier Tronoμl of 10× T4 RNA Ligase Buffer, 8 μl of 10 mM ATP or dATP, 2 μl of TdT (Takara, #2230B) or TT (NEB, #M0315L), 5 μl of 10 μM P5 Universal adapter were mixed in a total volume of 40 μl and incubated at 37°C. Aliquots

(6) High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seqNature CommunicationsMay 12, 2023Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, et al.0.5 µL 100 mM dATP (NEB, Cat # N0440S), 5 µL CoCl2. The TdT reaction kit was ordered from NEB (Cat # M0315S), including 10X TdT reaction buffer, TdT enzyme, and CoCl2. The dA tailing reaction mix was incubated

(7) Detecting recurrent passenger mutations in melanoma by targeted UV damage sequencingNature CommunicationsMay 11, 2023Kathiresan Selvam, Smitha Sivapragasam, Gregory M. K. Poon, John J. WyrickThe UV-irradiated DNA was sonicated, ligated to F1 adapter, and treated with terminal transferase (M0315S, NEB). The DNA was then digested with T4 endonuclease V (T4 PDG, M0308S, NEB) and AP endonuclease (M0282S,

(8) A massively parallel screening platform for converting aptamers into molecular switchesNature CommunicationsApril 24, 2023Alex M. Yoshikawa, Alexandra E. Rangel, Liwei Zheng, Leighton Wan, Linus A. Hein, Amani A. Hariri, Michael Eisenstein, H. Tom Sohapplications. Methods Materials Terminal transferase (TdT) was ordered from New England Biolabs (#M0315L). Ddel restriction enzyme was ordered from New England Biolabs (#R0175L). Cy3-labeled ddUTP (5-propargylamino-ddUTP-Cy3)

(9) CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryosGenome BiologyJanuary 19, 2023Youkui Huang, Bao-Qing Gao, Quan Meng, Liang-Zhong Yang, Xu-Kai Ma, Hao Wu, Yu-Hang Pan, Li Yang, Dong Li, Ling-Ling Chenmuc5.1 probe) or Red 650 (GCN4 and eppk1 probe) at the 3′ ends by Terminal Transferase (NEB, Cat. No. M0315L). In brief, reaction mixture for a 20 μL system includes the following: 1 μL probe mix (stock 100 μM),

(10) scPCOR-seq enables co-profiling of chromatin occupancy and RNAs in single cellsCommunications BiologyDecember 1, 2022Lixia Pan, Wai Lim Ku, Qingsong Tang, Yaqiang Cao, Keji ZhaoThermo Fisher Scientific (catalog no. 28906). Terminal Transferase was purchased from New England BioLabs (catalog no. M0315L). The human embryonic stem cell line H1 (WA01- lot WB35186 p30) was provided by WiCell Research Institute.

(11) ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situNature MethodsNovember 1, 2022Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, et al.Bioscience, NU-1707L) was added to the 3′ ends of the probes with Terminal Transferase (New England Biolabs, M0315L), which adds a single azido-dATP molecule. Then, the 5′ ends were phosphorylated with T4 Polynucleotide Kinase

(12) Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencingNature methodsNovember 1, 2022Kishi JY, Liu N, West ER, Sheng K, Jordanides JJ, Serrata M, Cepko CL, Saka SK, Yin P.dATP (NEB N0446S), 25 µM ddATP (Sigma GE27-2051-01) and 1,000 U ml−1 terminal transferase enzyme (NEB M0315L) for a 45-min incubation at 37 °C. After A-tailing, samples were washed in PBST for 3 × 1 min and stored

(13) Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machineryeLifeSeptember 5, 2022Andrea Volante, Juan Carlos Alonso, Kiyoshi Mizuuchidrug",Biotin-17-dCTP,Invitrogen,65601, "Chemical compound, drug",Terminal transferase,New England Biolabs,M0315, "Chemical compound, drug",Alexa Fluor 488 C5 maleimide,Thermo Fisher,A10254, "Chemical compound,

(14) Combining CRISPR–Cas12a with terminal deoxynucleotidyl transferase dependent reporter elongation for pathogen detection using lateral flow test stripsBiology Methods and ProtocolsJuly 27, 2022Nicole F Berghuis, Roos Mars-Groenendijk, Ruud W Busker, Armand Paauw, Hans C van Leeuwensignal for LFA read-out. Materials and methods Chemicals/reagents Engen LbCas12a (#M0653T) and TdT (#M0315L) were obtained from New England Biolabs (Ipswich, MA, USA) as were the matching CoCl2 (2.5 mM stock)

(15) Mre11-Rad50 oligomerization promotes DNA double-strand break repairNature communicationsMay 2, 2022Kissling VM, Reginato G, Bianco E, Kasaciunaite K, Tilma J, Cereghetti G, Schindler N, Lee SS, Guérois R, Luke B, et al.to 4 °C. Next, 1 µl of 10× tailing mix (2 µl 2 units/µl Terminal transferase (New England Biolabs, M0315S), 1 µl 10× NEBuffer 4, 1 µl 10 mM dCTP (Thermo Fisher, R0151), 6 µl sterile water) was added and incubated

(16) The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulationScience AdvancesApril 1, 2022Lorenzo Rinaldi et al.with ddUTP-biotin (NU-1619-biox, Jena Bioscience) using a DNA terminal transferase for 1 hour at 37°C (M0315, NEB). Beads were then successively washed with low-salt, high-salt, and LiCl buffer, and the chromatin

(17) Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencingGenome ResearchApril 1, 2022Samantha L. Sholes, Kayarash Karimian, Ariel Gershman, Thomas J. Kelly, Winston Timp, Carol W. Greidermolecular end, HMW genomic DNA (gDNA, see above) was first A-tailed using terminal transferase (NEB M0315) in a reaction by incubating 2 µg of gDNA, 1× terminal transferase buffer, 1× CoCl2, 5 mM dATP, and

(18) Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque modelMolecular Therapy - OncolyticsMarch 17, 2022Koji Terada et al.G-tailing solution containing 0.8 U terminal deoxynucleotidyl transferase (TdT) (New England Biolabs, M0315), 0.5 mM dGTP, 0.25 mM CoCl2, 5 U Qiagen RNase inhibitor (Qiagen, 129916), 5 U Porcine liver Ribonuclease Inhibitor

(19) Mapping atypical UV photoproducts in vitro and across the S. cerevisiae genomeSTAR ProtocolsMarch 1, 2022Kaitlynne A. Bohm, Smitha Sivapragasam, John J. Wyrick,New England Biolabs,M0201S Taq polymerase,VWR,2428-9844 Terminal Transferase Module,New England Biolabs,M0315L ,, Deposited data,Deposited data,Deposited data ,, Raw UVDE-seq data,"This study (Laughery et al.,

(20) Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factorsCell reportsFebruary 15, 2022Przanowska RK, Weidmann CA, Saha S, Cichewicz MA, Jensen KN, Przanowski P, Irving PS, Janes KA, Guertin MJ, Weeks KM, et al.,Life Technologies,Cat# P10144 Biotin-16-dUTP,Roche,Cat# 11093070910 Terminal Transferase,New England BioLabs,Cat# M0315S HaltTM Protease Inhibitor,Thermo Fisher Scientific,Cat# 78438 SUPERase●In™ RNase Inhibitor (20 U/μL),Ambion,Cat#

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